Produced:Article Title: OAS1 suppresses African swine fever virus replication by recruiting TRIM21 to degrade viral major capsid protein.
Article Snippet: .. The antibodies used in the study are as follows: Anti-ASFV P72 and P30 monoclo nal antibodies in mice were produced and stored in our laboratory; anti-RNase L antibody (Invitrogen, PA576443) was purchased from Invitrogen, anti-OAS1 antibody (Abcam,Ab272492) was purchased from Abcam, anti-STAT1 (CST, 14994S); anti-p-STAT1 (CST, 9167S), anti-STAT2 (CST, 72604S), anti-pSTAT2 (CST, 88410S), anti-GST (CST,2624S), and anti-G3BP1(CST, 61559S) were purchased from Cell Signaling Technology, antiDDX3X (Abcam, ab271002) and anti-DDX6 (Abcam, ab307418) were purchased from Abcam, anti-FLAG-Tag(CST, 14793S), anti-HA antibody (CST, 3724S), anti-His antibody (CST, 2365S), and anti-MYC antibody (CST, 2278S) were purchased from Cell Signaling Technology (USA); anti-β-actin antibody (Sigma, A1978) and anti-glyceraldehyde-3-phos phate dehydrogenase (GAPDH) antibody (Sigma, G8795) and DAPI (Sigma, D8417) were purchased from Sigma (USA), anti-rabbit IgG HampL (Alexa Fluor 488; Invitrogen, A11034), anti-mouse IgG HampL (Alexa Fluor 594; Invitrogen, A21125) were purchased from Invitrogen. .. MG-132 (MCE, HY-13259), 3-MA (MCE, HY-19312), and ML-60218 (MCE, HY-122122) were purchased from MedChemExpress; NH4Cl (Sigma, 09718), DMSO (Sigma, C6164), and sodium arsenite (Sigma-Aldrich, S7400) were purchased from Sigma-Aldrich.
Article Title: OAS1 suppresses African swine fever virus replication by recruiting TRIM21 to degrade viral major capsid protein
Article Snippet: .. Following adsorption, the viral culture medium was aspirated and replaced with a maintenance medium containing 2% FBS for further experiments. . Antibodies and reagents The antibodies used in the study are as follows: Anti-ASFV P72 and P30 monoclonal antibodies in mice were produced and stored in our laboratory; anti-RNase L antibody (Invitrogen, PA576443) was purchased from Invitrogen, anti-OAS1 antibody (Abcam,Ab272492) was purchased from Abcam, anti-STAT1 (CST, 14994S); anti-p-STAT1 (CST, 9167S), anti-STAT2 (CST, 72604S), anti-pSTAT2 (CST, 88410S), anti-GST (CST,2624S), and anti-G3BP1(CST, 61559S) were purchased from Cell Signaling Technology, anti-DDX3X (Abcam, ab271002) and anti-DDX6 (Abcam, ab307418) were purchased from Abcam, anti-FLAG-Tag(CST, 14793S), anti-HA antibody (CST, 3724S), anti-His antibody (CST, 2365S), and anti-MYC antibody (CST, 2278S) were purchased from Cell Signaling Technology (USA); anti-β-actin antibody (Sigma, A1978) and anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (Sigma, G8795) and DAPI (Sigma, D8417) were purchased from Sigma (USA), anti-rabbit IgG HampL (Alexa Fluor 488; Invitrogen, A11034), anti-mouse IgG HampL (Alexa Fluor 594; Invitrogen, A21125) were purchased from Invitrogen. .. MG-132 (MCE, HY-13259), 3-MA (MCE, HY-19312), and ML-60218 (MCE, HY-122122) were purchased from MedChemExpress ; NH4Cl (Sigma, 09718), DMSO (Sigma, C6164), and sodium arsenite (Sigma-Aldrich, S7400) were purchased from Sigma-Aldrich.
Adsorption:Article Title: OAS1 suppresses African swine fever virus replication by recruiting TRIM21 to degrade viral major capsid protein
Article Snippet: .. Following adsorption, the viral culture medium was aspirated and replaced with a maintenance medium containing 2% FBS for further experiments. . Antibodies and reagents The antibodies used in the study are as follows: Anti-ASFV P72 and P30 monoclonal antibodies in mice were produced and stored in our laboratory; anti-RNase L antibody (Invitrogen, PA576443) was purchased from Invitrogen, anti-OAS1 antibody (Abcam,Ab272492) was purchased from Abcam, anti-STAT1 (CST, 14994S); anti-p-STAT1 (CST, 9167S), anti-STAT2 (CST, 72604S), anti-pSTAT2 (CST, 88410S), anti-GST (CST,2624S), and anti-G3BP1(CST, 61559S) were purchased from Cell Signaling Technology, anti-DDX3X (Abcam, ab271002) and anti-DDX6 (Abcam, ab307418) were purchased from Abcam, anti-FLAG-Tag(CST, 14793S), anti-HA antibody (CST, 3724S), anti-His antibody (CST, 2365S), and anti-MYC antibody (CST, 2278S) were purchased from Cell Signaling Technology (USA); anti-β-actin antibody (Sigma, A1978) and anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (Sigma, G8795) and DAPI (Sigma, D8417) were purchased from Sigma (USA), anti-rabbit IgG HampL (Alexa Fluor 488; Invitrogen, A11034), anti-mouse IgG HampL (Alexa Fluor 594; Invitrogen, A21125) were purchased from Invitrogen. .. MG-132 (MCE, HY-13259), 3-MA (MCE, HY-19312), and ML-60218 (MCE, HY-122122) were purchased from MedChemExpress ; NH4Cl (Sigma, 09718), DMSO (Sigma, C6164), and sodium arsenite (Sigma-Aldrich, S7400) were purchased from Sigma-Aldrich.
Bioprocessing:Article Title: OAS1 suppresses African swine fever virus replication by recruiting TRIM21 to degrade viral major capsid protein
Article Snippet: .. Following adsorption, the viral culture medium was aspirated and replaced with a maintenance medium containing 2% FBS for further experiments. . Antibodies and reagents The antibodies used in the study are as follows: Anti-ASFV P72 and P30 monoclonal antibodies in mice were produced and stored in our laboratory; anti-RNase L antibody (Invitrogen, PA576443) was purchased from Invitrogen, anti-OAS1 antibody (Abcam,Ab272492) was purchased from Abcam, anti-STAT1 (CST, 14994S); anti-p-STAT1 (CST, 9167S), anti-STAT2 (CST, 72604S), anti-pSTAT2 (CST, 88410S), anti-GST (CST,2624S), and anti-G3BP1(CST, 61559S) were purchased from Cell Signaling Technology, anti-DDX3X (Abcam, ab271002) and anti-DDX6 (Abcam, ab307418) were purchased from Abcam, anti-FLAG-Tag(CST, 14793S), anti-HA antibody (CST, 3724S), anti-His antibody (CST, 2365S), and anti-MYC antibody (CST, 2278S) were purchased from Cell Signaling Technology (USA); anti-β-actin antibody (Sigma, A1978) and anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (Sigma, G8795) and DAPI (Sigma, D8417) were purchased from Sigma (USA), anti-rabbit IgG HampL (Alexa Fluor 488; Invitrogen, A11034), anti-mouse IgG HampL (Alexa Fluor 594; Invitrogen, A21125) were purchased from Invitrogen. .. MG-132 (MCE, HY-13259), 3-MA (MCE, HY-19312), and ML-60218 (MCE, HY-122122) were purchased from MedChemExpress ; NH4Cl (Sigma, 09718), DMSO (Sigma, C6164), and sodium arsenite (Sigma-Aldrich, S7400) were purchased from Sigma-Aldrich.
Staining:Article Title: IFN-α Induces Heterogenous ROS Production in Human β-Cells
Article Snippet: .. Tissues containing embedded islets were sectioned at 5μm thickness and stained by immunofluorescence using the following primary antibodies: anti-pSTAT2 (Cell Signaling Technology, CAT#88410, 1:200), anti-insulin (Dako, CAT#IR002, 1:10), anti-4-HNE (Abcam, CAT#ab48506, 1:100), and anti-8OHdG (Abcam, CAT#ab62623, 1:100). .. For secondary antibodies, Alexa Fluor 647 donkey anti-rabbit IgG (Invitrogen, CAT# A31573), Alexa Fluor 594 goat anti-mouse IgG (Invitrogen, CAT# A11032), and Alexa Fluor 488 goat anti-guinea pig (Invitrogen, CAT# A11073) IgG antibodies were used.
Immunofluorescence:Article Title: IFN-α Induces Heterogenous ROS Production in Human β-Cells
Article Snippet: .. Tissues containing embedded islets were sectioned at 5μm thickness and stained by immunofluorescence using the following primary antibodies: anti-pSTAT2 (Cell Signaling Technology, CAT#88410, 1:200), anti-insulin (Dako, CAT#IR002, 1:10), anti-4-HNE (Abcam, CAT#ab48506, 1:100), and anti-8OHdG (Abcam, CAT#ab62623, 1:100). .. For secondary antibodies, Alexa Fluor 647 donkey anti-rabbit IgG (Invitrogen, CAT# A31573), Alexa Fluor 594 goat anti-mouse IgG (Invitrogen, CAT# A11032), and Alexa Fluor 488 goat anti-guinea pig (Invitrogen, CAT# A11073) IgG antibodies were used.
other:Article Title: Porcine deltacoronavirus nucleocapsid protein antagonizes JAK-STAT signaling pathway by targeting STAT1 through KPNA2 degradation.
Article Snippet: Porcine deltacoronavirus (PDCoV) is an enteric pathogenic coronavirus that causes acute and severe watery diarrhea in piglets and has the ability of cross-species transmission, posing a great threat to swine production and public health.. The interferon (IFN)-mediated signal transduction represents an important component of virus-host interactions and plays an essential role in regulating viral infection.. Previous studies have suggested that multifunctional viral proteins encoded by coronaviruses antagonize the production of IFN via various means.
Western Blot:Article Title: Activation of APOBEC3 cytidine deaminases and endogenous retroviruses is integrated by MUC1-C in NSCLC cells
Article Snippet: .. Total lysates prepared from non-confluent cells were subjected to immunoblot analysis using anti-MUC1-C (HM-1630-P1ABX, 1:1000 dilution; Thermo Fisher Scientific, Waltham, MA, USA), anti-β-actin (A5441, 1:5000 dilution; Sigma-Aldrich, Burlington, MA, USA), anti-pSTAT1 (#9177S, 1:1000; Cell Signaling Technology (CST), Danvers, MA, USA), anti-STAT1 (#9172, 1:1000; CST); anti-pSTAT2 (#88410S, 1:1000; CST), anti-STAT2 (#72604, 1:1000; CST), anti-IRF9 (#76684, 1:1000; CST), anti-A3C (), anti A3F (), anti-A3G (), anti-IRF1 (#8478, 1:1000; CST), anti-cGAS (#15102, 1:1000; CST), anti-STING (#13647, 1:1000; CST), anti-MDA5 (#5321, 1:1000; CST) and anti-RIG-I (#3743, 1:1000; CST) as described [ , , ]. .. Chromatin immunoprecipitation (ChIP) was performed using control IgG (Santa Cruz Biotechnology), anti–MUC1-C (#MA5–11202, Thermo Fisher Scientific), anti-STAT1 (#ab109320, Abcam), anti-STAT2 (#8478, CST), anti-IRF9 (#76684, CST) and anti-IRF1 (#8478; CST) as described [ ].
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