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pstat2  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc pstat2
    Pstat2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 186 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+pstat2/Phospho-Stat2+(Tyr690)+Rabbit+mAb/pmc12817915-187-37-58
    Average 96 stars, based on 186 article reviews
    pstat2 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Produced:

    Article Title: OAS1 suppresses African swine fever virus replication by recruiting TRIM21 to degrade viral major capsid protein.
    Article Snippet: .. The antibodies used in the study are as follows: Anti-ASFV P72 and P30 monoclo nal antibodies in mice were produced and stored in our laboratory; anti-RNase L antibody (Invitrogen, PA576443) was purchased from Invitrogen, anti-OAS1 antibody (Abcam,Ab272492) was purchased from Abcam, anti-STAT1 (CST, 14994S); anti-p-STAT1 (CST, 9167S), anti-STAT2 (CST, 72604S), anti-pSTAT2 (CST, 88410S), anti-GST (CST,2624S), and anti-G3BP1(CST, 61559S) were purchased from Cell Signaling Technology, antiDDX3X (Abcam, ab271002) and anti-DDX6 (Abcam, ab307418) were purchased from Abcam, anti-FLAG-Tag(CST, 14793S), anti-HA antibody (CST, 3724S), anti-His antibody (CST, 2365S), and anti-MYC antibody (CST, 2278S) were purchased from Cell Signaling Technology (USA); anti-β-actin antibody (Sigma, A1978) and anti-glyceraldehyde-3-phos phate dehydrogenase (GAPDH) antibody (Sigma, G8795) and DAPI (Sigma, D8417) were purchased from Sigma (USA), anti-rabbit IgG HampL (Alexa Fluor 488; Invitrogen, A11034), anti-mouse IgG HampL (Alexa Fluor 594; Invitrogen, A21125) were purchased from Invitrogen. .. MG-132 (MCE, HY-13259), 3-MA (MCE, HY-19312), and ML-60218 (MCE, HY-122122) were purchased from MedChemExpress; NH4Cl (Sigma, 09718), DMSO (Sigma, C6164), and sodium arsenite (Sigma-Aldrich, S7400) were purchased from Sigma-Aldrich.

    Article Title: OAS1 suppresses African swine fever virus replication by recruiting TRIM21 to degrade viral major capsid protein
    Article Snippet: .. Following adsorption, the viral culture medium was aspirated and replaced with a maintenance medium containing 2% FBS for further experiments. . Antibodies and reagents The antibodies used in the study are as follows: Anti-ASFV P72 and P30 monoclonal antibodies in mice were produced and stored in our laboratory; anti-RNase L antibody (Invitrogen, PA576443) was purchased from Invitrogen, anti-OAS1 antibody (Abcam,Ab272492) was purchased from Abcam, anti-STAT1 (CST, 14994S); anti-p-STAT1 (CST, 9167S), anti-STAT2 (CST, 72604S), anti-pSTAT2 (CST, 88410S), anti-GST (CST,2624S), and anti-G3BP1(CST, 61559S) were purchased from Cell Signaling Technology, anti-DDX3X (Abcam, ab271002) and anti-DDX6 (Abcam, ab307418) were purchased from Abcam, anti-FLAG-Tag(CST, 14793S), anti-HA antibody (CST, 3724S), anti-His antibody (CST, 2365S), and anti-MYC antibody (CST, 2278S) were purchased from Cell Signaling Technology (USA); anti-β-actin antibody (Sigma, A1978) and anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (Sigma, G8795) and DAPI (Sigma, D8417) were purchased from Sigma (USA), anti-rabbit IgG HampL (Alexa Fluor 488; Invitrogen, A11034), anti-mouse IgG HampL (Alexa Fluor 594; Invitrogen, A21125) were purchased from Invitrogen. .. MG-132 (MCE, HY-13259), 3-MA (MCE, HY-19312), and ML-60218 (MCE, HY-122122) were purchased from MedChemExpress ; NH4Cl (Sigma, 09718), DMSO (Sigma, C6164), and sodium arsenite (Sigma-Aldrich, S7400) were purchased from Sigma-Aldrich.

    Adsorption:

    Article Title: OAS1 suppresses African swine fever virus replication by recruiting TRIM21 to degrade viral major capsid protein
    Article Snippet: .. Following adsorption, the viral culture medium was aspirated and replaced with a maintenance medium containing 2% FBS for further experiments. . Antibodies and reagents The antibodies used in the study are as follows: Anti-ASFV P72 and P30 monoclonal antibodies in mice were produced and stored in our laboratory; anti-RNase L antibody (Invitrogen, PA576443) was purchased from Invitrogen, anti-OAS1 antibody (Abcam,Ab272492) was purchased from Abcam, anti-STAT1 (CST, 14994S); anti-p-STAT1 (CST, 9167S), anti-STAT2 (CST, 72604S), anti-pSTAT2 (CST, 88410S), anti-GST (CST,2624S), and anti-G3BP1(CST, 61559S) were purchased from Cell Signaling Technology, anti-DDX3X (Abcam, ab271002) and anti-DDX6 (Abcam, ab307418) were purchased from Abcam, anti-FLAG-Tag(CST, 14793S), anti-HA antibody (CST, 3724S), anti-His antibody (CST, 2365S), and anti-MYC antibody (CST, 2278S) were purchased from Cell Signaling Technology (USA); anti-β-actin antibody (Sigma, A1978) and anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (Sigma, G8795) and DAPI (Sigma, D8417) were purchased from Sigma (USA), anti-rabbit IgG HampL (Alexa Fluor 488; Invitrogen, A11034), anti-mouse IgG HampL (Alexa Fluor 594; Invitrogen, A21125) were purchased from Invitrogen. .. MG-132 (MCE, HY-13259), 3-MA (MCE, HY-19312), and ML-60218 (MCE, HY-122122) were purchased from MedChemExpress ; NH4Cl (Sigma, 09718), DMSO (Sigma, C6164), and sodium arsenite (Sigma-Aldrich, S7400) were purchased from Sigma-Aldrich.

    Bioprocessing:

    Article Title: OAS1 suppresses African swine fever virus replication by recruiting TRIM21 to degrade viral major capsid protein
    Article Snippet: .. Following adsorption, the viral culture medium was aspirated and replaced with a maintenance medium containing 2% FBS for further experiments. . Antibodies and reagents The antibodies used in the study are as follows: Anti-ASFV P72 and P30 monoclonal antibodies in mice were produced and stored in our laboratory; anti-RNase L antibody (Invitrogen, PA576443) was purchased from Invitrogen, anti-OAS1 antibody (Abcam,Ab272492) was purchased from Abcam, anti-STAT1 (CST, 14994S); anti-p-STAT1 (CST, 9167S), anti-STAT2 (CST, 72604S), anti-pSTAT2 (CST, 88410S), anti-GST (CST,2624S), and anti-G3BP1(CST, 61559S) were purchased from Cell Signaling Technology, anti-DDX3X (Abcam, ab271002) and anti-DDX6 (Abcam, ab307418) were purchased from Abcam, anti-FLAG-Tag(CST, 14793S), anti-HA antibody (CST, 3724S), anti-His antibody (CST, 2365S), and anti-MYC antibody (CST, 2278S) were purchased from Cell Signaling Technology (USA); anti-β-actin antibody (Sigma, A1978) and anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (Sigma, G8795) and DAPI (Sigma, D8417) were purchased from Sigma (USA), anti-rabbit IgG HampL (Alexa Fluor 488; Invitrogen, A11034), anti-mouse IgG HampL (Alexa Fluor 594; Invitrogen, A21125) were purchased from Invitrogen. .. MG-132 (MCE, HY-13259), 3-MA (MCE, HY-19312), and ML-60218 (MCE, HY-122122) were purchased from MedChemExpress ; NH4Cl (Sigma, 09718), DMSO (Sigma, C6164), and sodium arsenite (Sigma-Aldrich, S7400) were purchased from Sigma-Aldrich.

    Staining:

    Article Title: IFN-α Induces Heterogenous ROS Production in Human β-Cells
    Article Snippet: .. Tissues containing embedded islets were sectioned at 5μm thickness and stained by immunofluorescence using the following primary antibodies: anti-pSTAT2 (Cell Signaling Technology, CAT#88410, 1:200), anti-insulin (Dako, CAT#IR002, 1:10), anti-4-HNE (Abcam, CAT#ab48506, 1:100), and anti-8OHdG (Abcam, CAT#ab62623, 1:100). .. For secondary antibodies, Alexa Fluor 647 donkey anti-rabbit IgG (Invitrogen, CAT# A31573), Alexa Fluor 594 goat anti-mouse IgG (Invitrogen, CAT# A11032), and Alexa Fluor 488 goat anti-guinea pig (Invitrogen, CAT# A11073) IgG antibodies were used.

    Immunofluorescence:

    Article Title: IFN-α Induces Heterogenous ROS Production in Human β-Cells
    Article Snippet: .. Tissues containing embedded islets were sectioned at 5μm thickness and stained by immunofluorescence using the following primary antibodies: anti-pSTAT2 (Cell Signaling Technology, CAT#88410, 1:200), anti-insulin (Dako, CAT#IR002, 1:10), anti-4-HNE (Abcam, CAT#ab48506, 1:100), and anti-8OHdG (Abcam, CAT#ab62623, 1:100). .. For secondary antibodies, Alexa Fluor 647 donkey anti-rabbit IgG (Invitrogen, CAT# A31573), Alexa Fluor 594 goat anti-mouse IgG (Invitrogen, CAT# A11032), and Alexa Fluor 488 goat anti-guinea pig (Invitrogen, CAT# A11073) IgG antibodies were used.

    other:

    Article Title: Porcine deltacoronavirus nucleocapsid protein antagonizes JAK-STAT signaling pathway by targeting STAT1 through KPNA2 degradation.
    Article Snippet: Porcine deltacoronavirus (PDCoV) is an enteric pathogenic coronavirus that causes acute and severe watery diarrhea in piglets and has the ability of cross-species transmission, posing a great threat to swine production and public health.. The interferon (IFN)-mediated signal transduction represents an important component of virus-host interactions and plays an essential role in regulating viral infection.. Previous studies have suggested that multifunctional viral proteins encoded by coronaviruses antagonize the production of IFN via various means.

    Western Blot:

    Article Title: Activation of APOBEC3 cytidine deaminases and endogenous retroviruses is integrated by MUC1-C in NSCLC cells
    Article Snippet: .. Total lysates prepared from non-confluent cells were subjected to immunoblot analysis using anti-MUC1-C (HM-1630-P1ABX, 1:1000 dilution; Thermo Fisher Scientific, Waltham, MA, USA), anti-β-actin (A5441, 1:5000 dilution; Sigma-Aldrich, Burlington, MA, USA), anti-pSTAT1 (#9177S, 1:1000; Cell Signaling Technology (CST), Danvers, MA, USA), anti-STAT1 (#9172, 1:1000; CST); anti-pSTAT2 (#88410S, 1:1000; CST), anti-STAT2 (#72604, 1:1000; CST), anti-IRF9 (#76684, 1:1000; CST), anti-A3C (), anti A3F (), anti-A3G (), anti-IRF1 (#8478, 1:1000; CST), anti-cGAS (#15102, 1:1000; CST), anti-STING (#13647, 1:1000; CST), anti-MDA5 (#5321, 1:1000; CST) and anti-RIG-I (#3743, 1:1000; CST) as described [ , , ]. .. Chromatin immunoprecipitation (ChIP) was performed using control IgG (Santa Cruz Biotechnology), anti–MUC1-C (#MA5–11202, Thermo Fisher Scientific), anti-STAT1 (#ab109320, Abcam), anti-STAT2 (#8478, CST), anti-IRF9 (#76684, CST) and anti-IRF1 (#8478; CST) as described [ ].



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    Image Search Results


    IFIT3 knockdown relieved the intestinal inflammatory response and inhibited the activation of the STAT1/2 pathway in DSS-induced UC mice. ( A and B ) Comparison of colon transduction efficacy in mice between intraperitoneal injection of saline and AAV9 using paraffin sections ( A ) and the IVIS Lumina III imaging system ( B ). Scale bar = 100 µm. ( C ) Comparison of colon length between AAV9-shNC- and AAV9-shIFIT3-treated UC mice. ( D ) Weight changes in the two mouse groups. ( E ) Representative images and histological score of H&E staining in colon tissues from the two mouse groups. Scale bar = 100 µm (top) and 25 µm (bottom). ( F ) mRNA levels of IFIT3, TNF-α, IL-1β and IL-6 in colon tissues from the two mouse groups. ( G ) Protein expressions of STAT1, pSTAT1, STAT2, pSTAT2 and IFIT3 in colon tissues from the two mouse groups. Data was presented as mean ± SD (n = 5). * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Journal of Inflammation Research

    Article Title: Downregulation of IFIT3 Relieves the Inflammatory Response in Ulcerative Colitis via Selectively Regulating Macrophage M1 Polarization and the STAT1/2 Signaling Pathway

    doi: 10.2147/JIR.S542033

    Figure Lengend Snippet: IFIT3 knockdown relieved the intestinal inflammatory response and inhibited the activation of the STAT1/2 pathway in DSS-induced UC mice. ( A and B ) Comparison of colon transduction efficacy in mice between intraperitoneal injection of saline and AAV9 using paraffin sections ( A ) and the IVIS Lumina III imaging system ( B ). Scale bar = 100 µm. ( C ) Comparison of colon length between AAV9-shNC- and AAV9-shIFIT3-treated UC mice. ( D ) Weight changes in the two mouse groups. ( E ) Representative images and histological score of H&E staining in colon tissues from the two mouse groups. Scale bar = 100 µm (top) and 25 µm (bottom). ( F ) mRNA levels of IFIT3, TNF-α, IL-1β and IL-6 in colon tissues from the two mouse groups. ( G ) Protein expressions of STAT1, pSTAT1, STAT2, pSTAT2 and IFIT3 in colon tissues from the two mouse groups. Data was presented as mean ± SD (n = 5). * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: The PVDF membranes were incubated with primary antibodies against IFIT3 (1:3000; #15201-1-AP, Proteintect), STAT1 (1:1000; # R25799 , Zenbio, Chengdu, China), pSTAT1 (1:1000; #bsm-52209R, Bioss, Beijing, China), STAT2 (1:1000; #R381419 Zenbio), pSTAT2 (1:1000; #bs-3428R, Bioss), and GAPDH (1:1000; #GB15002-100, Servicebio) overnight at 4 °C and then incubated with HRP-labeled secondary antibodies at room temperature for 1 hour.

    Techniques: Knockdown, Activation Assay, Comparison, Transduction, Injection, Saline, Imaging, Staining

    IFIT3 knockdown repressed macrophage M1 polarization by inhibiting the STAT1 pathway in vitro. ( A and B ) mRNA and protein levels of IFIT3 in untreated THP‐1 macrophages or treated with 100 ng/mL LPS or 100 ng/mL LPS + 20 ng/mL IFN‐γ or 20 ng/mL IL‐4. ( C ) mRNA levels of IFIT3 in 100 ng/mL LPS-stimulated macrophages treated with siNC or two siRNAs targeting IFIT3 (siIFIT3-1 and siIFIT3-2). ( D ) mRNA levels of TNF-α, IL-1β and IL-6 in 100 ng/mL LPS-stimulated macrophages treated with siNC and siIFIT3-2. ( E ) Protein levels of STAT1, pSTAT1, STAT2, pSTAT2 and IFIT3 in each group. ( F ) Representative gating and the co-expression of CD86 and CD68 in untreated (M0) and 100 ng/mL LPS-stimulated macrophages (M1) by flow cytometry. ( G ) Representative gating and the co-expression of CD68 and CD86 in 100 ng/mL LPS-stimulated macrophages treated with siNC and siIFIT3-2 by flow cytometry. Data was presented as mean ± SD from 3 independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Journal: Journal of Inflammation Research

    Article Title: Downregulation of IFIT3 Relieves the Inflammatory Response in Ulcerative Colitis via Selectively Regulating Macrophage M1 Polarization and the STAT1/2 Signaling Pathway

    doi: 10.2147/JIR.S542033

    Figure Lengend Snippet: IFIT3 knockdown repressed macrophage M1 polarization by inhibiting the STAT1 pathway in vitro. ( A and B ) mRNA and protein levels of IFIT3 in untreated THP‐1 macrophages or treated with 100 ng/mL LPS or 100 ng/mL LPS + 20 ng/mL IFN‐γ or 20 ng/mL IL‐4. ( C ) mRNA levels of IFIT3 in 100 ng/mL LPS-stimulated macrophages treated with siNC or two siRNAs targeting IFIT3 (siIFIT3-1 and siIFIT3-2). ( D ) mRNA levels of TNF-α, IL-1β and IL-6 in 100 ng/mL LPS-stimulated macrophages treated with siNC and siIFIT3-2. ( E ) Protein levels of STAT1, pSTAT1, STAT2, pSTAT2 and IFIT3 in each group. ( F ) Representative gating and the co-expression of CD86 and CD68 in untreated (M0) and 100 ng/mL LPS-stimulated macrophages (M1) by flow cytometry. ( G ) Representative gating and the co-expression of CD68 and CD86 in 100 ng/mL LPS-stimulated macrophages treated with siNC and siIFIT3-2 by flow cytometry. Data was presented as mean ± SD from 3 independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Article Snippet: The PVDF membranes were incubated with primary antibodies against IFIT3 (1:3000; #15201-1-AP, Proteintect), STAT1 (1:1000; # R25799 , Zenbio, Chengdu, China), pSTAT1 (1:1000; #bsm-52209R, Bioss, Beijing, China), STAT2 (1:1000; #R381419 Zenbio), pSTAT2 (1:1000; #bs-3428R, Bioss), and GAPDH (1:1000; #GB15002-100, Servicebio) overnight at 4 °C and then incubated with HRP-labeled secondary antibodies at room temperature for 1 hour.

    Techniques: Knockdown, In Vitro, Expressing, Flow Cytometry

    UPA inhibits inflammation by silencing IFIT3 in LPS-stimulated macrophages. ( A ) mRNA levels of IFIT3, TNF-α, IL-1β and IL-6 in 100 ng/mL LPS-stimulated macrophages with untreated or treated with 100 nM and 400 nM UPA. ( B ) Protein levels of STAT1, pSTAT1, STAT2, pSTAT2 and IFIT3 among cell-groups. ( C ) Schematic diagram of relation among UPA, IFIT3 and STAT1/2 pathways. Data was presented as mean ± SD from 3 independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Journal: Journal of Inflammation Research

    Article Title: Downregulation of IFIT3 Relieves the Inflammatory Response in Ulcerative Colitis via Selectively Regulating Macrophage M1 Polarization and the STAT1/2 Signaling Pathway

    doi: 10.2147/JIR.S542033

    Figure Lengend Snippet: UPA inhibits inflammation by silencing IFIT3 in LPS-stimulated macrophages. ( A ) mRNA levels of IFIT3, TNF-α, IL-1β and IL-6 in 100 ng/mL LPS-stimulated macrophages with untreated or treated with 100 nM and 400 nM UPA. ( B ) Protein levels of STAT1, pSTAT1, STAT2, pSTAT2 and IFIT3 among cell-groups. ( C ) Schematic diagram of relation among UPA, IFIT3 and STAT1/2 pathways. Data was presented as mean ± SD from 3 independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Article Snippet: The PVDF membranes were incubated with primary antibodies against IFIT3 (1:3000; #15201-1-AP, Proteintect), STAT1 (1:1000; # R25799 , Zenbio, Chengdu, China), pSTAT1 (1:1000; #bsm-52209R, Bioss, Beijing, China), STAT2 (1:1000; #R381419 Zenbio), pSTAT2 (1:1000; #bs-3428R, Bioss), and GAPDH (1:1000; #GB15002-100, Servicebio) overnight at 4 °C and then incubated with HRP-labeled secondary antibodies at room temperature for 1 hour.

    Techniques: